Accumulation and translocation of lead in vegetables through intensive use of organic manure and mineral fertilizers with wastewater

In many countries with wastewater irrigation and intensive use of fertilizers (minerals and organics), heavy metal deposition by crops is regarded as a major environmental concern. A study was conducted to determine the impact of mineral fertilizers, cow manure, poultry manure, leaf litter, and sugarcane bagasse on soil’s trace Pb content and edible parts of vegetables. It also evaluated the risk of lead (Pb) contamination in water, soil, and food crops. Six vegetables (Daucus carota, Brassica oleracea, Pisum sativum, Solanum tuberosum, Raphanus sativus, and Spinacia oleracea) were grown in the field under twelve treatments with different nutrient and water inputs. The lead concentrations in soil, vegetables for all treatments and water samples ranged from 1.038–10.478, 0.09346–9.0639 mg/kg and 0.036–0.26448 mg/L, The concentration of lead in soil treated with wastewater in treatment (T6) and vegetable samples was significantly higher, exceeding the WHO’s permitted limit. Mineral and organic fertilizers combined with wastewater treatment reduced lead (Pb) concentrations in vegetables compared to wastewater application without organic fertilizers. Health risk indexes for all treatments except wastewater treatment (T6) were less than one. Pb concentrations in mineral fertilizers, cow manure, poultry manure, leaf litter, and sugarcane bagasse treated were determined to pose no possible risk to consumers.


Experimental site
The experimental work was carried out in Chak # 89 S.B, situated in District Sargodha, central Punjab, Pakistan.The geographical coordinates for this study were 32.083° N and 72.6719° E.An average of 410 mm of rain falls in the area each year.It is situated at a height of around 190 m above sea level, with a mean temperature of 23.8 °C being recorded.

Treatments
The study comprised 12 treatment combinations of four levels of organic manure (control, cow manure at a rate of 20 t ha −1 , poultry manure at a rate of 3 t ha −1 , leaf litter at the rate of 50 t ha −1 , sugarcane bagasse ash at a rate of 40 t ha −1 ) and one inorganic fertilizers (nitrate) at a rate of 100 kg ha −1 ) with irrigation of canal water and wastewater.

Field preparation
The field of study was ploughed fully and transversely furrowed using a mold sheet plow, and cross-scouring was performed with a tractor.The softened ground was set out in an experimental layout.Next, planking was completed, and the soil was brought to a fine tilth.Paths and canals were also prepared in 2.4 × 1.8 m 2 plots according to the design.Cow manure, Poultry manure, leaf litter manure, and sugarcane bagasse manure were utilized at a rate of 20 t ha −1 , 3 t ha −1 , 50 t ha −1 , and 40 t ha −1 , and spread uniformly in a bed size of 2.4 × 1.8 m 2 .Its quantity was calculated and applied before sowing.The recommended dose of nitrate for vegetables was 100 kg ha −1 .Experiments were carried out on selected vegetables for two yrs.A completely randomized design was used for the trial, with 12 treatments, five replicates, and six vegetables (12 × 5 × 6) (Table 1).The research consisted of field experiments in the work location and under the same environmental conditions.At maturity, fruit parts of vegetables were used for Pb analyses.

Irrigation
Vegetables were appropriately irrigated with canal water and wastewater at the intervals of 7-14 days for 2 yrs.
www.nature.com/scientificreports/Sampling Water, soil and vegetables sampling.Five replicates of wastewater and canal water samples that were used for irrigation purpose were collected and transferred in to the water analysis lab and stored at 4 °C before analysis.Before manure addition, five replicates of soil samples were taken at 0-30 cm depths for physical analysis.After adding manure and nitrate, soil samples with five replicates were collected for Pb and physical analysis at 0-30 cm depths by digging out a stainless steel auger.After that, the soil samples were dried in the air and stored in plastic bags for future study.
The mature edible portions of vegetables were collected randomly from the study site.Root vegetables consist of carrot (Daucus carota), radish (Raphanus sativus), potato (Solanum tuberosum).Leaf containing vegetables spinach (Spinacia oleracea) and cauliflower (Brassica oleracea).Seed containing vegetables (Pisum sativum).Vegetable samples were collected randomly, with five replicates from each plot irrigated with canal water and wastewater.Samples were washed through the water to remove soil particles, and vegetable samples were dry in air at 80 °C to constant mass.Dried samples were crushed using a mortar and pestle.After that, the samples were air-dried and kept in sealed paper bags with labels.
Pb analysis of water, soil, and vegetables samples.To investigate the level of Pb in water, 20 ml of water samples were digested with pure HNO 3 (15 ml) until colorless at 80 °C.After filtration using whatman Filters #42 paper, distilled water was added to make the volume up to 50 ml.The dry powdered soil and food crop samples (1 g) were digested with 5 ml of concentrated HNO 3 and aqua regia (15 ml, 70% Conc.HNO 3 and 65% HClO 4 ; 2:1) and heated until white fumes appeared at 80 °C.After filtering, the digested mixture was diluted with purified water to make a final amount of 50 ml.
The lead level in water, soil, and vegetable samples was analyzed using a calibrated atomic absorption spectrophotometer (Shimadzu Co., Ltd., Japan).The Samples were evaluated against the National Institute of Standard Technology's Standard Reference Material (SRM) 1570 for lead to ensure precise and accurate results.The conditions for this process involved a wavelength of 283.3 nm, a slit width of 0.7 nm, a lamp current of ten mA, an airflow rate of 15 L/min, an acetylene flow rate of 2.0 L/min, and a flame height of 7 mm.Each sample was tested for Pb three times.All results were in line with worldwide standards.

Quality control
All the chemical reagents used for this study were sourced from Sigma Aldrich, Merck (Germany), and BDH (U.K.).These experiments used only Pyrex glassware.This glassware was meticulously cleaned with Max liquid detergent and then oven-dried at 100 °C for 1 h to ensure cleanliness.The pollution load index (PLI) is calculated using Eq. ( 1) 18 .
M is the metal content (mg/kg), I.S. is the tested soil metal content, and RS. is the soil metal reference value.PLI 0 or < 1 implies no metal pollution, while PLI > 1 indicates high metal pollution and reduced soil value.

Bio-concentration factor
The bio-concentration factor (BCF) compares soil and an edible plant metal concentration is calculated through Eq. (2).
Soil enrichment factor (E.F.) Soil enrichment factor (E.F) is utilized for estimating the accumulation of heavy metals concentration in soil as determined by the Eq. ( 3).
The reference values for lead (Pb) in soil (8.15 mg/kg) and vegetable (3 mg/kg) samples.

Daily intake of lead (DIM)
The daily intake of heavy metal (DIM) was calculated by applying this Eq.( 4).
where C is the vegetable metal concentration; Cf is the conversion factor, 0.085.D is the daily food consumption, 0.345 kg/person/day and B is the normal adult weight, 65 kg.

Health risk index (HRI)
The health risk index for metal consumption from contaminated vegetables was calculating using the Eq. ( 5).R f D for the lead was 0.004

Statistical analysis
The result from each variable was statistically examined after the samples were analyzed with Microsoft Excel and Minitab 16.The means of soil, crop and water samples were compared using a three-factor factorial design (three-way ANOVA), and when the p-value was less than 0.05, it was concluded that the difference between the means had statistical significance.The mean of different treatments was compared through analysis of variance.Correlation analysis was used to find out Pb movement from soil to vegetables.The mean values for each treatment were compared using an analysis of variance.

Statement regarding plant guidelines
Research regarding this manuscript was conducted inside the Department of Botany, University of Sargodha, Sargodha, Pakistan and during this research no animal or plant was harmed and no work was performed on new germline or new species.Furthermore, the plants were treated after the approval and guidelines from the departmental permission and standard methods were used to analyze the plant materials.

Concentration of lead (Pb) in the irrigated water
Lead values in water showed significant (P < 0.05) variations in yr, water type, and water type × yr (Table 3).The Pb mean for Crop lead (Pb) versus treatment, vegetables and yr (Table 4).The concentration of lead contents ranged from 0.0329 to 0.0473 mg/L in freshwater and 0.1500-0.2847mg/L in wastewater during both yrs (Table 5).

Concentration of lead (Pb) in soil irrigated with wastewater
The data for analysis of variance for soil lead (Pb) value showed highly significant variation (P < 0.05) among treatments, vegetables, yrs, and their interactions like treatments × vegetables, treatment × yrs, vegetables × yrs, treatments × vegetables × yrs (Table 6).Soil lead (Pb) content varied for various treatments from 1.476 to 10.407 mg/kg during both yrs (Fig. 1A).The higher lead (Pb) values (10.48 mg/kg) were analyzed in Daucus carota during 2nd yr and treatment T 6 (Clean soil + wastewater treatment).In comparison, the lower lead (Pb) values (1.48 mg/kg) were analyzed in Raphanus sativus during 2nd yr in treatment T 3 (Leave compost at the rate of 50 t ha −1 + canal water irrigation) (Fig. 1B).The results show the mean concentration of lead (Pb) in www.nature.com/scientificreports/vegetables and soil during both yrs for all treatments (Table 7).Mean value of lead (Pb) for all treatments in soil was 4.560 mg/kg and 5.476 mg/kg for 1st and 2nd yrs (Table 8).

Concentration of lead (Pb) in vegetables irrigated with wastewater
The data for analysis of variance for vegetable lead (Pb) value showed significant variation (P < 0.05) among treatments, vegetables, yr, and their interactions like treatments × vegetables, treatments × yrs, vegetables × yrs, treatments × vegetables × yrs (Table 6).Vegetables lead (Pb) content varied for various treatments from 0.097 to 9.40 mg/kg during both years in soil (Fig. 1A,B).The higher lead (Pb) values (9.40 mg/kg) were analyzed in Spinacia oleracea during 2nd yr and treatment T 5 (Nitrate at 100 kg ha −1 + canal water irrigation).The lower lead (Pb) values (0.097 mg/kg) were analyzed in Raphanus sativus during 2nd yr and in treatment T 2 (Poultry manure at the rate of 3 t ha −1 + canal water irrigation) (Fig. 1C,D).Mean value of lead (Pb) for all treatments in vegetables was 3.244 mg/kg and 3.74 mg/kg for 1st and 2nd yrs (Table 8).

The pollution load index (PLI) of lead (Pb) in respective samples of vegetables
The lead PLI value ranged from 0.0729 to 1.525 depending on the vegetables, yr, and treatment.A lower level of PLI (0.0723) was observed in Raphanus sativus during 1st yr in treatment T 2 .In contrast, a higher PLI (1.525) value was observed in Daucus Carota during 1st yr of treatment T 11 (Fig. 2A,B).According to treatments, higher mean value of PLI for lead (Pb) in treatment T 6 during 2nd yr was 1.08 (Table 9) while according to vegetables, the mean value of lead PLI was higher in S. tuberosum was 0.938 (Table 10).

Bio-concentration factor of lead (Pb) in respective samples of vegetables
The value of the lead bio-concentration factor varied from 0.057 to 1.28, and this variation depended on the vegetables, yr, and treatment.Spinacia oleracea had a higher value of BCF was 1.28 at treatment T 8 in 1st yr.In comparison, Raphanus sativus had a lower BCF value at treatment T 3 (Leave compost at the rate of 50 t ha −1 + canal water irrigation) in 1st yr (Fig. 2C).Higher mean value of BCF for lead in treatment recorded at T 1 in S. oleracea 2nd yr (Fig. 2D).Higher mean value of BCF for lead in treatment T 11 (Nitrate at the rate of 100 kg ha −1 + wastewater irrigation) and 1st yr was 0.889 (Table 11).The average value of lead BCF was higher in S. Oleracea was 1.059 (Table 12).

The enrichment factor (EF) of lead (Pb) in respective samples of vegetables
The enrichment factor (EF) value for lead varied from 0.156 to 3.49.In the 1st yr in treatment T 8 , Spinacia oleracea had maximum EF value (3.49) (Fig. 3A).In contrast, Raphanus sativus had the minimum EF value (0.156) during 1st yr in treatment T 3 (Leave compost at the rate of 50 t ha −1 + canal water irrigation) (Fig. 3B).
The highest mean EF value for lead was noted (4.144), for the 1st yr in treatment T 11 (Nitrate at the rate of 100 kg ha −1 + wastewater irrigation) (Table 13).In the case of S. oleracea, the average value of lead EF (3.19) was higher (Table 14).

Daily intake (DIM) of lead (Pb) in respective samples of vegetables
Lead DIM was measured from 0.00004 to 0.0040 mg/kg/day.The lowest value for daily intake of Pb, 0.00004 mg/ kg/day, was found in Raphanus sativus during 1st yr in treatment T 3 (Leave compost at the rate of 50 t ha −1 + canal water irrigation), but the highest value for daily intake of Pb 0.0040 mg/kg/day was found in Spinacia oleracea during 1st yr in treatment T 6 (Cow manure at the rate of 20 t ha −1 + wastewater irrigation) (Fig. 3C).The highest value for daily intake of Pb was found in D. carota during 2nd yr in treatment T 5 (Fig. 3D).The higher mean value of DIM for lead in treatment T 9 during 1st yr was 1.186 mg/kg/day (Table 15).The mean value of daily intake for lead was higher in S. Oleracea was 0.0030 mg/kg/day (Table 16).

Health risk index (HRI) of lead (Pb) in respective samples of vegetables
Lead HRI was measured in the range of 0.0105-1.022.The lowest HRI value (0.0105) was found in Raphanus sativus 1st yr treatment T 3 (Leave compost at the rate of 50 t ha −1 + canal water irrigation), but the higher HRI (1.022) was found in Spinacia oleracea in 1st yr treatment T 7 (Cow manure at the rate of 20 t ha −1 + wastewater irrigation) (Fig. 4A,B).Higher mean value of health risk index for lead in treatment T 6 and 2nd yr was 0.733 (Table 17).Mean value for health risk index of lead was greatest in S. Oleracea was 0.579 (Table 18).

Scatter plot analysis for lead (Pb) concentration in soils and vegetables irrigation with waste
Scatter plot analysis was conducted to examine the lead concentration in soils and vegetables for each treatment.
A positive correlation was identified between concentration of lead (Pb) in the soil and in the vegetables.A notably significant positive correlation was observed between soils and vegetables.However, the regression analysis also indicated a non-significant (P > 0.05) value for the lead (Pb) in the soil, and similarly, a nonsignificant (P > 0.05) regression in the vegetable samples (Fig. 4C,D).

Lead (Pb) levels in wastewater
In this current analysis, the concentrations of lead in wastewater and canal water were higher, ranging from 0.044 to 00.21 mg/L, compared to the previously reported values 18 .observed that lead contents (7.15 mg/L) in wastewater were significantly higher than the current analysis finding.
Lead is primarily consumed by plants through the topmost layer of soil.The presence of lead (Pb) in the water sources used for crop irrigation may have resulted in the decreased availability of lead (Pb) for plant absorption 19 .External factors such as pollution from heavy traffic or the presence of lead (Pb) in crop irrigation water sources for crops may have impacted the availability of lead (Pb) to plants.Lead accessibility for plant uptake may also be decreased by environmental contamination brought on by vehicle emissions.

Lead (Pb) levels in soil after treatment with various fertilizers and wastewater
In one of study observed that lead contents in soil range from 1.95 to 4.9 mg/kg, which had similar mean values of lead (Pb) content in treatments T 2 , T 3 , T 4 , T 5 , T 7 , T 8 , T 10 (1st yr) and treatments T 1 , T 2, T 3 , T 4 , (2nd yr) while more significant in all other treatments 20 .
Irrigating soil with canal water and wastewater significantly increases the concentration of lead (Pb).This revealed that the consistent use of wastewater cause to the accumulation of lead (Pb) in the soil.Results found that wastewater-irrigated soil increased the accumulation of lead contents in the soil's upper layer.In a study it was described soil treated with cattle manure showed a lead (Pb) content between 3.6 and 5.9 mg/kg, similar

Lead (Pb) levels in vegetables after treatment with various fertilizers and wastewater
Applying municipal solid waste (MSW), treatments increased the soil's lead (Pb) levels by 97.2%.Applying 200 t of manure per hectare, Pb in the soil increased by roughly 14-278% compared to soil where no manure was used 22 .Mixing plant waste with manure reduced the effectiveness of removing Pb from polluted soils, suggesting it's a beneficial way to absorb these elements and improve soil fertility.Treatments of decomposed poultry manure with pine waste decreased the remaining lead (Pb) percentage in soil from 294 mg kg −1 (1:0) to 276 mg kg −1 when applied at 10 t per hectare and 20 t per hectare 23 .It was observed that the lead (Pb) content in wastewater-irrigated vegetables increased by 28 mg/kg, showing a higher lead (Pb) content in all treatments and yrs in our current data 9 .
Using wastewater significantly increased the lead (Pb) levels in the soil that produced all these vegetables (p 0.05).Lead (Pb) contents in soil and vegetables increased after Pb-containing wastewater was applied 9 .It was reported that the average amount of lead (Pb) in the edible parts of vegetables ranged between 1.8 and 11 mg/kg, demonstrating higher lead build-up than our current data 24 .Lead (Pb) contents in spinach range from 1.0 mg/kg, which were lower in all treatments of spinach as compared to present finding.The lead (Pb) levels in vegetables irrigated with wastewater ranged from 32.2 to 34.2 mg/kg, which exceeds our present data for these vegetables 25 .
Lead contamination of the environment is being researched because lead entry into the food chain may affect human health and vegetable metabolism.The growing need for agricultural land increases nutrient movement, decreasing the soil's essential nutrients.Using organic materials is vital for the long-lasting health of intensive systems.Many studies have shown the positive effects of applying organic matter to soil properties.Heavy use of cow manure significantly impacts the accumulation of Pb in the soil.The notable increase emphasizes the role of cow manure in large-scale farming.The lead (Pb) content decreases with the application of cow manure average lead (Pb) content in various food crops fertilized with poultry manure (varying from 0.41 to 11.4 mg per kg) was significantly higher than the current results 26 .In soil treated with poultry manure, the available lead content for plants was 10%, compared to 14% lead value in the untreated soil 27 .
Pb spread and transfer to the environment can be reduced by compost as compared to fresh manure.Applying compost manure in polluted soils has been shown to lower heavy Pb contamination and has proven beneficial and effective.According to WHO, the maximum safe level of lead (Pb) for human use is 0.3 mg per kg of dry weight 28 .Lead (Pb) levels in the edible parts of plants varied between 0.09346 and 9.0639.The lead content in these plant parts was above the safe limit.Lead levels decreased when manure was added, while they increased when wastewater was used.Ecological exposure, compost, and wastewater treatment are farming fields' primary lead sources (Pb).Large clusters of industrial facilities, vehicle emissions, re-suspended roadway sand, and petrol engine systems all play a part in lead absorption.
A higher pollution load index (0.62 mg/kg) was reported compared to present study 29 .In most studies, lead (Pb), a major pollutant of concern, often exceeded a value of 1, The Pollution Load Index (PLI) increase can be attributed to various human activities.It was reported that the Pollution Load Index (PLI) for lead (Pb) was within the range of 0.49-0.61,similar results observed in treatments T 3 and T 4 in the 1st yr and T 1 in the 2nd yr of our current study 30 .The PLI value experienced a decrease when a combination of fertilizers, namely poultry   www.nature.com/scientificreports/manure, cow manure, and NPK, was applied.The PLI value decreased and was observed to be less than 1 in all treatments throughout both years, except treatment T 6 in the 2nd yr, which involved wastewater irrigation.The bio-concentration factor of lead irrigated with canal water and wastewater, (Pb) in vegetables ranged from 0.18 to 0.20 and 0.18 to 0.19.These values indicated a lower average compared to the findings of our current study.The Bio-concentration Factor (BCF) in vegetables irrigated with wastewater was higher, with observed values ranging from 0.096 and 0.211 mg/kg 31 .Upon treating the soil with composted cattle manure at a rate of 10 t per hectare, the Bio-concentration Factor (BCF) value of lead (Pb) in plants was reduced to 7.6 mg/kg, ultimately similar to the control treatment (10.8 mg kg 1 ) 23 .The application of manure reduced the total Pb content in the soil.Manure could reduce lead (Pb) availability in soil through complex structures that form between organic matter and Pb.
Alterations with high-pH organic substances may further decrease the absorption of lead (Pb) 23 .It was observed the enrichment factor (EF) value for lead (Pb) was 0.53, which is lower compared to our current study.The enrichment factor value in vegetables was 2.187, which is higher, and was reported for the accumulation in the edible parts of vegetables grown in soil irrigated with wastewater and fertilized with inorganic fertilizers 32 .Plants that transfer lead from the soil to their edible parts have an enrichment factor (EF) greater than 1.These consumable plant portions accumulate more lead (Pb) than the soil.Metals with a high enrichment factor (EF) value can quickly move into the consumable parts of plants 33 .
The daily Pb intake was calculated as 0.345 kg of vegetable for a 65 kg standard body mass.The daily average values for metal consumption was identified by 34 .The USEPA published suggested consumption for reference value for many metals 35 .Soil irrigated with wastewater had higher value of daily intake of Pb as compared to current research.Amount of daily intake of lead was reported 8.15 to 75.06 mg/day 36 and similar results were analyzed by 37 .Results reported that lower value of daily intake of Pb for wastewater irrigated soil as compared to current research.
Value of daily intake of Pb recorded higher (3.51 mg/day/kg) in body weight as compared to the present data 31 .Humans, especially children, can develop mental and neurological problems as a result of lead poisoning.Higher value of the health risk index than our current research was reported 38 .The health risk index (HRI) levels in canal water and wastewater-irrigated soil ranged from 8.83 to 9.09 mg/kg, exceeding the acceptable limits 39 .

Conclusions
In response to dwindling fresh water resources, farmers have increasingly relied on wastewater for enhancing agricultural productivity.Wastewater also contains vital nutrients for plant growth, in addition to harmful chemicals.The results indicate that soils were enriched with lead (Pb).Applying organic fertilizers reduced the lead (Pb) concentration in the soil, which was generated by the usage of wastewater and its transfer to edible crops.This study examined the possible health effects resulting from lead (Pb) pollution of soil and vegetables.The addition of wastewater resulted in a health risk index (HRI) of less than 1, meaning that lead (Pb) pollution has no negative consequences.Treatment T 6 (wastewater + Soil without manure) contains the highest amount of chromium in soil among all treatments.However, analyses have shown that both soil and crops contain lead concentrations in the safe levels recommended by the Food and Agriculture Organization (FAO) and the World Health Organization (WHO).In order to prevent lead poisoning in soils and crops and potential health risks in the vicinity of the study zone, it is imperative to regularly monitor water pollution and treatment.Addition of mineral and organic fertilizers can reduce the Pb accumulation in vegetables.

Figure 1 .
Figure 1.(A-D) Difference in the value of Lead (Pb) (mg/kg) in soils, Concentration of lead in soil for year-1 (A), Concentration of lead in soil for year-2 (B), Concentration of lead in vegetables for year-1 (C), Concentration of lead in vegetables for year-2 (D).

Figure 2 .
Figure 2. (A-D) Difference in the value of Lead (Pb) (mg/kg), Difference in the value of PLI in year-1 (A), Difference in the value of PLI in Year-2 (B), Difference in the value of BCF in year-1 (C), Difference in the value of BCF in year-2 (D).

Figure 3 .
Figure 3. (A-D) Difference in EF and DIM, Difference in the value of EF in year-1 (A), Difference in the value of EF in year-2 (B), Difference in the value of DIM in year-1 (C), Difference in the value of DIM in year-2 (D).

Figure 4 .
Figure 4. (A-D) Correlation and value difference between concentration of lead in vegetables and soil for all treatments during both years.Difference in the value of HRI in year-1 (A), Difference in the value of HRI in year-2 (B), Correlation between vegetables and soil (C), Correlation between vegetables and soil in year-2 (D).

Table 1 .
List of Treatments to selected vegetables at Sargodha.

Table 2 .
Selected vegetables are grown in Sargodha from waste water and canals water with fertilizers.The edible portions of the vegetables were taken for Pb analysis.

Table 5 .
Mean concentrations of lead (Pb) (mg/L) in diverse source of water during both years.

Table 7 .
Mean concentrations of lead (Pb) (mg/kg) in vegetables and soil during both years for all treatments.

Table 8 .
Mean Concentrations of lead (Pb) (mg/kg) in vegetables and soil for both years.

Table 9 .
Mean for analysis of Value of PLI on the base of treatments in both years.

Table 10 .
Mean Value of PLI in vegetables for all treatments during both years.

Table 11 .
Mean Value of BCF on the base of treatments in both years.

Table 12 .
Mean Value of BCF in vegetables for all treatments during both years.

Table 13 .
Mean Value of EF on the base of treatments in both years.

Table 14 .
Mean Value of EF in vegetables for all treatments during both years.

Table 15 .
Mean Value of DIM on the base of treatments in both years.

Table 16 .
Mean Value of DIM in vegetables for all treatments during both years.

Table 17 .
Mean Value of HRI in vegetables on the base of treatments in both years.

Table 18 .
Mean Value of HRI in vegetables for all treatments during both years.